| Continental Tire The Americas, LLC | 3432 | Manufacturing |
| Walgreens Distribution Center | 1380 | Regional Distribution |
| SSM Health Good Samaritan Hospital Center | 2073 | Healthcare |
| Crossroads Community Hospital | 290 | Healthcare |
| National Railway Equipment Company | 207 | Manufacturing |
| Mt. Vernon City School District 80 | 283 | Education |
| Rend Lake College | 261 | Education |
| Mt. Vernon Township High School | 171 | Education |
| City of Mt. Vernon | 129 | Municipality |
| Jefferson County | 178 | Local Unit of Government |
| Kroger | 155 | Retail |
| Peoples National Bank | 102 | Banking |
| Spero | 193 | Service |
| Mt. Vernon Neon Sign | 38 | Manufacturing |
| NAPA Distribution Center | 90 | Distribution |
| Medline | 77 | Healthcare |
| Durham Bus Service | 84 | Transportation |
| Orthopedic Center of Southern Illinois | 78 | Medical Billing |
| Magnum Steelworks | 91 | Manufacturing |
